2003_4380

Laboratory Information:

Click here to to download a zipped program to convert your dissolved oxygen text files into usuable data for an Excel spreadsheet. Follow the instructions below for conversion of the files:

- run the conversion program (SWKon.exe) to convert the original text file into a .csv file.

- change the extension from .csv to .txt.

- open Excel and check on delimited and then check tab, semi colon and comma

- your dissolved oxygen data will be in column B of the spreadsheet

Course Description:

Bioreactor design is an integral part of bioprocess engineering. Bioreactors are controlled environments for microbiological and biochemical reactions to produce value-added products or to treat waste streams. Typical processes include fermentations to produce antibiotics, wine and yoghurt, enzymatic reactors to create ingredients such as high fructose corn syrup and natural bio-systems such as composting operations and biofilters. Design of bioreactors requires integration of microbiology, biochemistry, process engineering and economic analysis. The aim of bioreactor design is to produce product(s) with specific quality attributes at minimum cost.

ENGG*4380 introduces biological engineering students to modelling and design of batch and continuous bioreactors based on biological growth kinetics and mass balances. Additional design topics include: gas-liquid mass transfer for aeration and agitation; instrumentation and control.

Prerequisite: ENGG*3830 Bio-process Engineering

Objectives: Students who successfully complete this course will be able to:

1. Describe and specify reactors used in industrial bioprocesses.

2. Develop mathematical models for bioreactors and analyse their dynamic behaviour dynamic and steady state).

3. Specify operating parameters for optimal performance of ideal bioreactor systems.

4. Design complete bioreactor systems including instrumentation and control components.

Topics (times indicated are approximate):

• Introduction to Reactor Design

• Kinetics of Bioreactions (8 hours):

Growth kinetics of microorganisms

- exponential growth - Monod model and substrate balances

- yield coefficients and maintenance requirements

- lag phase kinetics- Dean and Hinshelwood model

- stationary phase

Product formation kinetics

- growth associated

- non-growth associated

Enzyme kinetics (review)

• Mass transfer in bioreactors (5 hours):

Aeration - mass transfer correlations

Agitation - mass transfer, power and holdup correlations

• Ideal reactors (8 hours)

Batch reactors (closed and fed-batch)

Continuous reactors:

Plug Flow Tubular Reactor (PFTR) - mass balances

Continuous Stirred Tank Reactor (CSTR) - mass balances

Reactors for biomass growth

Reactors in series

Recycle

• Instrumentation and Control (2 hours)

• Industrial Operations (6 hours)

- large-scale reactors - equipment & services

- GMP and process validation

- economics - process CAD software

• Residence time distributions to assess non-idealities (4 hours)

- models for non-ideal reactors

- tanks-in-series

- axial dispersion

Textbook:
Bioprocess Engineering Basic Concepts (2nd edition) 2002. Michael L. Shuler and Fifret Kargi, Prentice Hall, Upper Saddle River, NJ

Additional learning aids
A set of student notes has been prepared to supplement lectures for this course. The notes include the main points of lecture material but not all of the details which will be discussed. The notes include references to appropriate sections of the textbook as well as to reference books which follow.

Biochemical Engineering. Harvey W. Blanch and Douglas S. Clark. Marcel Dekker, Inc. 1997.
Biochemical Engineering Fundamentals. 2nd edition. James E. Bailey and David F Ollis. McGraw-Hill 1986. (TP 248.3B34 1986)
Chemical Reaction Engineering. 2nd edition. O. Levenspiel. John Wiley and Sons, Inc., New York 1972.
Basic Bioreactor Design. K. van't Riet and J. Tramper. Marcel Dekker, Inc., New York 1991.

Assessment:

Assignments: There will be three assignments that include calculations for different aspects of bioreactor analysis and design. Tutorial time is available to work on the assignments, consult with the instructor and make use of software tools on the School's network. Assignments are due on the following dates: September 26th, October 17th and November 7th.

Laboratory assignments: There will be three laboratory assignments. You will work in groups to complete the "hands on" work in the laboratory but you will submit individual reports. The format for each report will be outlined in the experiment outline. The laboratory assignments are due on the following dates: October 10th, October 31st and November 21st.

Design project: The design project is a term-long assignment allowing students to apply knowledge gained in this course to an area of interest. Design projects will conducted in groups of two. There will be two interim meetings with the instructor and each design group to discuss progress. The meetings will be scheduled during the tutorial time during the weeks of October 6th and November 10th. The final designs will be presented in the lecture time on November 27th and the final report is due on November 28th.

Assignments (3) - 15%
Laboratory design assignments(3) - 25%
Group design project - 20%
Final examination - 40% (02 December 2003 11:30 am - 1:30 pm)
TOTAL - 100%

Note:
Requests for academic consideration because of illness or of a compassionate nature must be made in writing and accompanied by certification whenever possible.